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The ABCs Of Gene Cloning

by Dominic W.S. Wong

Publisher: Springer/Star Educational Books

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Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning. Tables of Contents:- Preface Part One. Fundamentals of Genetic Processes 1. Introductory Concepts 1.1 What is DNA and What is a Gene 1.2 What is Gene Cloning? 1.3 Cell Organizations 1.4 Heredity Factors and Traits 1.5 Mitosis and Meiosis 1.6 Relating Genes to Inherited Trait 1.7 Why Gene Cloning? Review 2. Structures of Nucleic Acids 2.1 3’-OH and 5’-P Ends 2.2 Purine and Pyrimidine Bases 2.3 Complementary Base Pairing 2.4 Writing a DNA Molecule 2.5 Describing DNA sizes 2.6 Denaturation and Renaturation 2.7 Ribonucleic Acid Review 3. Structures of Proteins 3.1 Amino Acids 3.2 The Peptide Bond 3.3 Structural Organization 3.4 Postranslational Modification 3.5 Enzymes Review 4. The Genetic Process 4.1 From Genes to Proteins 4.2 Transcription 4.3 Translation 4.4 The Genetic Code 4.5 Why Present a Sequence Using the Coding Strand? 4.6. The Reading Frame 4.7 DNA Replication 4.8 The Replicon and Replication Origin 4.9 Relating Replication to Gene Cloning Review 5. Organization of Genes 5.1 The Lactose Operon 5.2 Control of Transcription 5.2.1 The Transcription Start Site and Termination Site 5.2.2 When Does Transcription Start or Stop? 5.3. Control of Translation 5.3.1 Ribosome Binding Site and Start Codon 5.3.2 Translation Termination Site 5.4 The Tryptophan Operson 5.4.1 Co-repressor 5.4.2 Attenuation 5.4.3 Hybrid Promoters 5.5 The Control System in Eukaryotic Cells 5.5.1 Transcriptional Control 5.5.2 Introns and Exons 5.5.3 Capping and Tailing 5.5.4 Ribosome Binding Sequence 5.5.5 Monocistronic and Polycistronic Review 6. Reading the Nucleotide Sequence of a Gene 6.1 The E. coli dut gene 6.2 The human bgn gene 6.2.1 Reading the genomic sequence 6.2.2 Reading the cDNA sequence Part Two. Techniques and Strategies of Gene Cloning 7. Enzymes Used in Cloning 7.1 Restriction Enzymes 7.2 Ligase 7.3 DNA Polymerases 7.3.1 E. coli DNA Polymerase I 7.3.2 Bacteriophage T4 and T7 polymerase 7.3.3 Reverse Transcriptase 7.3.4 RNA Polymerase 7.4 Phosphatase and kinase Review 8. Techniques Used in Cloning 8.1 DNA Isolation 8.2 Gel Electrophoresis 8.2.1 Agarose Gel Electrophoresis 8.2.2 Polyacrylamide Gel Electrophoresis 8.3 Wester Blot 8.4 Southern Transfer 8.5 Colony Blot 8.6 Hybridization 8.7 Immunological Techniques 8.8 DNA Sequencing 8.9 Polymerase Chain Reaction 8.10 Site-Directed Mutagenesis 8.11 Non-radioactive Detection Methods 90 Review 9. Cloning Vectors for Introducing Genes into Host Cells 9.1 Vectors for Bacterial Cells 9.1.1 Plasmid Vecors 9.1.2 Bacteriophage Vectors 9.1.3 Cosmids 9.1.4 Phagemids 9.2 Yeast Cloning Vectors 9.2.1 The 2ì Circle 9.3 Vectors for Plant Cells 9.3.1 Binary Vector System 9.3.2 Cointegrative Vector System 9.3.3 Genetic Markers 9.3.4 Plant Specific Promoters 9.4 Vectors for Mammalian Cells 9.4.1 SV40 Viral Vectors 9.4.2 Direct DNA Transfer 9.4.3 Insect Baculovirus 9.4.4 Retrovirus Review 10. Transformation 10.1 Calcium Salt Treatment 10.2 Electroporation 10.3 Agrobacterium Infection 10.4 The Biolistic Process 10.5 Viral Transfection 10.6 Microinjection 10.7 Nuclear Transfer Review 11. Isolating Genes for Cloning 11.1 The Genome Library 11.2 The cDNA Library 11.3 Choosing The Right Cell Types for mRNA Isolation Review Part Three. Impact of Gene Cloning - Applications in Agriculture 12 Improving Tomato Quality by Antisense RNA 12.1 Antisense RNA 12.2 A Strategy for Engineering Tomatoes with Review 13. Transgenic Crops Engineered with Insecticidal Activity 13.1 Bacillus thuringiensis Toxins 13.2 Cloning of the cry gene into cotton plants 13.2.1 Modifying the cry gene 13.2.2 The Intermediate Vector 13.2.3 Transformation by Agrobacterium Review 14. Transgenic Crops Conferred with Herbicide Resistance 14.1 Glyphosate 14.2 Cloning of the aroA gene Review 15. Growth Enhancement in Transgenic Fish 15.1 Gene Transfer in Fish 15.2 Cloning Salmons with a Chimeric Growth Hormone Gene Review Part Four. Impact of Gene Cloning - Applications in Medicine and Related Areas 16. Microbial Production of Recombinant Human Insulin 16.1 Structure and Action of Insulin 16.2 Cloning Human Insulin Gene Review 17. Finding Disease-Causing Genes 17.1 Genetic Linkage 17.1.1 Frequency of Recombination 17.1.2 Genetic Markers 17.2 Positional Cloning 17.2.1 Chromosome Walking 17.2.2 Chromosome Jumping 17.2.3 Yeast Artificial Chromosome 17.3 Exon Amplification 17.4 Isolation of the Mouse Obese Gene Review 18. Human Gene Therapy 18.1 Physical Chemical Methods 18.2 Biological Methods 18.2.1 Life Cycle of Retroviruses 18.2.2 Construction of a Safe Retrovirus Vector 18.3 Gene Treatment of Severe Combined Immune Deficiency 18.4 Therapeutic Vaccines 18.4.1 Construction of DNA Vaccines 18.4.2 Methods of Delivery Review 19. Gene Targeting 19.1 Recombination 19.2 Replacement Targeting Vectors 19.3 Gene Targeting Without Selectable Markers 19.3.1 The PCR Method 19.3.2 The Double-Hit Method 19.3.3 The Cre/loxP Recombination 19.4 Gene Targeting for Xenotransplants Review 20. DNA Typing 20.1 Variable Number Tandem Repeats 20.2 Polymorphism Analysis Using VNTR Markers 20.3 Single-locus and Multi-locus Probes 20.4 Paternity Case Analysis 20.5 Short Tandem Repeat Markers 20.5.1 The Combined DNA Index System 20.6 Mitochondrial DNA Sequence Analysis Review 21. Transpharmers - Bioreactors for Pharmaceutical Products 21.1 General Procedure for Production of Transgenic Animals 21.2 Transgenic Sheep for á1-Antitrypsin Review 22. Animal Cloning 22.1 Cell Differentiation 22.2 Nuclear Transfer 22.3 The Cloning of Dolly Review 23. Human Genome Sequencing 23.1 Genetic Maps 23.1.1 DNA Markers 23.1.2 Pedigree Analysis 23.2 Physical Maps 23.2.1 Sequence Tagged Sites 23.2.2 Radiation Hybridization 23.2.3 Clone Libraries 23.2.4 Bacteria Artificial Chromosome Vector 23.3 Comprehensive Integrated maps 23.4 Strategies for Genome Sequencing 23.4.1 Hierarchical Shotgun Sequencing 23.4.2 Whole-Genome Shotgun Sequencing Review References Index

Details of the book

Book :
The ABCs Of Gene Cloning
Book ID :
74741
Author :
Dominic W.S. Wong
ISBN 13 :
9788184890143
Year of Publication :
2008
Publisher :
Springer/Star Educational Books
Binding :
Softcover
Weight :
1 kg

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